LOCUS       sequence-73431-e        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS1.2 (JW640)
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..891
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(151..170)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     761..780
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
       61 ttagagtctc atattcactc tcaactcgat cgaggcatga ttgaacaaga tggattgcac
      121 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      181 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      241 gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg
      301 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      361 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      421 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
      481 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
      541 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
      601 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccac
      661 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
      721 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
      781 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
      841 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
      901 tggggttcgg actctagcta gagtcaagca gatcgttcaa acatttggca ataaagtttc
      961 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     1021 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     1081 at
//
