LOCUS       sequence-73435-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS2.1 (FF570)
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(332..677)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(369..389)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggctcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atggggccgc caatatgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatcc tctagattcg
      301 cttgtagaga aaaatcacag ctcgtccccc gtgttctctc caaatgaaat gaacttcctt
      361 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
      421 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
      481 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga
      541 tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct
      601 tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc
      661 tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg atatttttgg
      721 agtagacaag tgtgtcgtgc tccaccatgt tgacgaagat tttcttcttg tcattgagtc
      781 gtaagagact ctgtatgaac tgttcgccag tctttacggc gagttctgtt aggtcctcta
      841 tttgaatctt tgactccatg gcctttgatt cagtgggaac taccttttta gagactccaa
      901 tctctattac ttgccttggt ttgtgaagca agccttgaat cgtccatact ggaatagtac
      961 ttctgatctt gagaaatata tctttctct
//
