LOCUS       sequence-73444-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS3.2 (FF574)
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      263..904
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(926..946)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(926..944)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(965..981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(965..981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(978..1000)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    989..1005
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcacctcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggtgatc ctagctttcg ttcgtatcat cggtttcgac aacgttcgtc
      301 aagttcaatg catcagtttc attgcgcaca caccagaatc ctactgagtt tgagtattat
      361 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
      421 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
      481 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
      541 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
      601 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta
      661 tgcttattca ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga
      721 atacaagtat gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca
      781 gaatccttgt cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag
      841 ttgagtatga aaatattttt taatgcattt tatgacttgc caattgattg acaacatgca
      901 tcaatcggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      961 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaac
//
