LOCUS       sequence-73449-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS4.1 (FF571)
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     291..307
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     291..307
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggctcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atggggccgc caatatgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatcc tctagaacta
      301 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gaccgacgag
      361 tcagtaataa acggcgtcaa agtggttgca gccggcacac acgagtcgtg tttatcaact
      421 caaagcacaa atacttttcc tcaacctaaa aataaggcaa ttagccaaaa acaactttgc
      481 gtgtaaacaa cgctcaatac acgtgtcatt ttattattag ctattgcttc accgccttag
      541 ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt cttctataaa acaataccca
      601 aagagctctt cttcttcaca attcagattt caatttctca aaatcttaaa aactttctct
      661 caattctctc taccgtgatc aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg
      721 attttgtttt cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct
      781 tagatcgaag acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg
      841 tttcatagat atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc
      901 gatttgtgat ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtag
      961 attaatctga gtttttctga t
//
