LOCUS       sequence-73451-e        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS4.1 (FF571)
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        40..58
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     40..57
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(91..798)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtacca tttaggtgac actatagaat
       61 atcactagta agctagcttg catgccggtc ctgctgagcc tcgacatgtt gtcgcaaaat
      121 tcgccctgga cccgcccaac gatttgtcgt cactgtcaag gtttgacctg cacttcattt
      181 ggggcccaca tacaccaaaa aaatgctgca taattctcgg ggcagcaagt cggttacccg
      241 gccgccgtgc tggaccgggt tgaatggtgc ccgtaacttt cggtagagcg gacggccaat
      301 actcaacttc aaggaatctc acccatgcgc gccggcgggg aaccggagtt cccttcagtg
      361 agcgttatta gttcgccgct cggtgtgtcg tagatactag cccctggggc acttttgaaa
      421 tttgaataag atttatgtaa tcagtctttt aggtttgacc ggttctgccg ctttttttaa
      481 aattggattt gtaataataa aacgcaattg tttgttattg tggcgctcta tcatagatgt
      541 cgctataaac ctattcagca caatatattg ttttcatttt aatattgtac atataagtag
      601 tagggtacaa tcagtaaatt gaacggagaa tattattcat aaaaatacga tagtaacggg
      661 tgatatattc attagaatga accgaaaccg gcggtaagga tctgagctac acatgctcag
      721 gttttttaca acgtgcacaa cagaattgaa agcaaatatc atgcgatcat aggcgtctcg
      781 catatctcat taaagcaggg atcccgcatc agatctatca aacacataga tttacgaaga
      841 tctctaacat taaatcatca gagagataag tttagggagc aagctctttc gcttacacag
      901 agaatcacag aggaaagaga ctttaagaaa actacaactt ttttgaccac tgtgatttct
      961 ctctgcaagc gaaagtactt aatcaccaac acagtagatt gatatatttg ggtttatttg
     1021 tgagaagaag aattcactag tggatcccgc actcgagtgt taatcagaaa aac
//
