LOCUS       sequence-73452-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS4.1 (FF571)
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             105..656
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      676..928
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    952..974
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
       61 ttagagtctc atattcactc tcaactcgat cgaggggatc taccatgagc ccagaacgac
      121 gcccggccga catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca
      181 accactacat cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggagt
      241 ggacggacga cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg
      301 gcgaggtcgc cggcatcgcc tacgcgggtc cctggaaggc acgcaacgcc tacgactgga
      361 cggccgagtc caccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc
      421 tctacaccca cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca
      481 tcgggctgcc caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg
      541 gcatgctgcg ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc
      601 tggacttcag cctgccggtg ccgccccgtc cggtcctgcc cgtcaccgaa atctgatgac
      661 ccctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
      721 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
      781 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
      841 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
      901 cgcgcggtgt catctatgtt actagatcga ccggcatgca agctgataac gttacaccac
      961 aatatatcct gccaagatct aattccgggg atcggaaatc cagaagcccg agaggttgcc
     1021 gcc
//
