LOCUS       sequence-73458-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded H2O2 probe for ratiometric and fluorescence 
            lifetime imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Bilan DS, Pase L, Joosen L, Gorokhovatsky AY, Ermakova YG, Gadella 
            TW, Grabher C, Schultz C, Lukyanov S, Belousov VV
  TITLE     HyPer-3: a genetically encoded H2O2 probe with improved performance 
            for ratiometric and fluorescence lifetime imaging.
  JOURNAL   ACS Chem Biol. 2012 Dec 20.
  PUBMED    23256573
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2012 Dec 20."
COMMENT     Sequence Label: pQE-30-HyPer3
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(75..169)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     609..628
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     868..889
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 ccaagctagc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      121 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagctcagct
      181 aattaagctt ttaaaccgcc tgttttaaaa ctttatcgaa atggccatcc attcttgcgc
      241 ggatggcctc tgccagctgc tcatagcggc tgcgcagcgg tgagccagga cgataaacca
      301 gaccaatagt gcggcgtggt tccggcttaa tgcacggcag ataaacaacc ccatcgcgtt
      361 tgcgctccgg cggcacagcc agcgctggca gtaaagtgat cccgctacct gccgccacca
      421 tgttgcgcag agtttccagg ctggtcgcgc ggaagtgtgt atcttcatcc gccccggctt
      481 caaaacagaa accggtgccg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      541 tgaagccgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      601 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
      661 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
      721 agcacttcag gccgtagccg agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      781 cggtggtgca gatcagcttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      841 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      901 ccccggtgaa cagctcctcg cccttgctgc cggtgccacc gctaccgcca tccacgttgt
      961 acagctcgtc c
//
