LOCUS       sequence-73462-e        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human ELL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-ELL2
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..38
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(206..217)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 catgctgcat gccagactac aaggacgatg atgacaagct cgacatggcg gcggggggga
       61 cagggggcct gcgggaggag cagcgctatg ggctgtcgtg cggacggctg gggcaggaca
      121 acatcaccgt actgcatgtg aagctcaccg agacggcgat ccgggcgctc gagacttacc
      181 agagccacaa gaatttaatt ccttttcgac cttcaatcca gttccaagga ctccacgggc
      241 ttgtcaaaat tcccaaaaat gatcccctca atgaagttca taactttaac ttttatttgt
      301 caaatgtggg caaagacaac cctcagggca gctttgactg catccagcaa acattctcca
      361 gctctggagc ctcccagctc aattgcctgg ggtttataca agataaaatt acagtgtgtg
      421 caacaaacga ctcgtatcag atgacacgag aaagaatgac ccaggcagag gaggaatccc
      481 gcaaccgaag cacaaaagtt atcaaacccg gtggaccata tgtagggaaa agagtgcaaa
      541 ttcggaaagc acctcaagct gtttcagata cagttcctga gaggaaaagg tcaaccccca
      601 tgaaccctgc aaatacaatt cgaaagacac atagcagcag caccatctct cagaggccat
      661 acagggacag ggtgattcac ttactggccc tgaaggccta caagaaaccg gagctacttg
      721 ctagactcca gaaagatggt gtcaatcaaa aagacaagaa ctccctggga gcaattctgc
      781 aacaggtagc caatctgaat tctaaggacc tctcatatac cttaaaggat tatgttttta
      841 aagagcttca aagagactgg cctggataca gtgaaataga cagacggtca ttggagtcag
      901 tgctctctag aaaactaaat ccgtctcaga atgctgcagg caccagccgt tcagaatctc
      961 ctgtatgttc tagtagagat gctgtatctt ctctcagaaa cggcttttgg attcagagtt
     1021 tattgatctt atgaataaaa aaagccgaat atctcactga cgacagagta cacacactaa
     1081 atggtcattg atcccaccag tgaaaatccg ctgcagctcc actgcccctg g
//
