LOCUS       sequence-73469-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Lager I, Looger LL, Hilpert M, Lalonde S, Frommer WB
  TITLE     Conversion of a putative Agrobacterium sugar-binding protein into a 
            FRET sensor with high selectivity for sucrose.
  JOURNAL   J Biol Chem. 2006 Oct 13. 281(41):30875-83.
  PUBMED    16912038
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 Oct 13. 281(41):30875-83."
COMMENT     Sequence Label: pRSET FlipSuc-90u
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(251..272)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     600..619
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgtcgacatc cttgttgccg gcggcaagcc acagccggta ctggctgaac tgttcgctgg
       61 aagaagacgg catggtgacg agattgacct tgtgaccggt ctctttctcg aaagcggcga
      121 tgcgttcgcg gaaaaacgcg acgttcttgc cggtcgaatt tgcggcaatg gaaagttcgg
      181 ccgcctcagc ggcgcctccg gtaccaccct tgtacagctc gtccatgccg agagtgatcc
      241 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      301 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      361 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      421 ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt
      481 ggctgatgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      541 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      601 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      661 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
      721 cgccccaggt ca
//
