LOCUS       sequence-73474-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Peroxide-insensitive version of HyPer to be used as a pH-control. 
            Can be applied as a pH sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, 
            Terskikh AV, Lukyanov S
  TITLE     Genetically encoded fluorescent indicator for intracellular hydrogen
            peroxide.
  JOURNAL   Nat Methods. 2006 Apr;3(4):281-6.
  PUBMED    16554833
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2006-04"; volume: "3(4)"; pages: "281-6"
COMMENT     Sequence Label: pC1-HyPer-C199S
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     561..580
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     820..841
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
       61 gtggtatggc tgattatgat cagttatcta gatccggtgg atcccgggcc cgcggtaccg
      121 tcgactgcag aattcgaagc ttttaaaccg cctgttttaa aactttatcg aaatggccat
      181 ccattcttgc gcggatggcc tctgccagct gctcatagcg gctgcgcagc ggtgagccag
      241 gacgataaac caggccaata gtgcggcgtg gttccggctt aatgcacggc agataaacaa
      301 ccccatcgcg tttgcgctcc ggcggcacag ccagcgctgg cagtaaagtg atcccgctac
      361 ctgccgccac catgttgcgc agagtttcca ggctggtcgc gcggaagtgt gtatcttcat
      421 ccgccccggc ttcaaaacag aaaccggtgc cgttgtactc cagcttgtgc cccaggatgt
      481 tgccgtcctc cttgaagccg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      541 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      601 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      661 ggtagcgggc gaagcacttc aggccgtagc cgagggtggt cacgagggtg ggccagggca
      721 cgggcagctt gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc
      781 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      841 ggatgggcac caccccggtg aacagctcct cgcccttgct gccggtgcca ccgctaccgc
      901 catccacgtt gtacagctcg tccatgccga
//
