LOCUS       sequence-73476-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Flp driven by the constitutive promoter pE from 
            phage P2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pE-FLP
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             54..62
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ttagatctca atagtattta gtttagatgt agattgttta gtgcttgatg tactaaggag
       61 gttgtatgcc acaatttgat atattatgta aaacaccacc taaggtgctt gttcgtcagt
      121 ttgtggaaag gtttgaaaga ccttcaggtg agaaaatagc attatgtgct gctgaactaa
      181 cctatttatg ttggatgatt acacataacg gaacagcaat caagagagcc acattcatga
      241 gctataatac tatcataagc aattcgctga gtttcgatat tgtcaataaa tcactccagt
      301 ttaaatacaa gacgcaaaaa gcaacaattc tggaagcctc attaaagaaa ttgattcctg
      361 cttgggaatt tacaattatt ccttactatg gacaaaaaca tcaatctgat atcactgata
      421 ttgtaagtag tttgcaatta cagttcgaat catcggaaga agcagataag ggaaatagcc
      481 acagtaaaaa aatgcttaaa gcacttctaa gtgagggtga aagcatctgg gagatcactg
      541 agaaaatact aaattcgttt gagtatactt cgagatttac aaaaacaaaa actttatacc
      601 aattcctctt cctagctact ttcatcaatt gtggaagatt cagcgatatt aagaacgttg
      661 atccgaaatc atttaaatta gtccaaaata agtatctggg agtaataatc cagtgtttag
      721 tgacagagac aaagacaagc gttagtaggc acatatactt ctttagcgca aggggtagga
      781 tcgatccact tgtatatttg gatgaatttt tgaggaattc tgaaccagtc ctaaaacgag
      841 taaataggac cggcaattct tcaagcaata aacaggaata ccaattatta aaagataact
      901 tagtcagatc gtacaataaa gctttgaaga aaaatgcgcc ttattcaatc tttgctataa
      961 aaaatggccc aaaatctcac attgg
//
