LOCUS       sequence-73492-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression empty vector (yeast).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_gRNA_handle_RPR1t
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..21)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    394..441
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagta
       61 tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg
      121 gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa
      181 tgggataata tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata
      241 agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag
      301 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc
      361 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc
      421 tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac
      481 tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc
      541 ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata
      601 taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt
      661 ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag
      721 agtgaatcga aaacaatacg aaaatgt
//
