LOCUS       sequence-73493-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes human-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pCMV_dCas9_VP64
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..99)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccgttgtgt gatcagtttg gcgttcagca gctgccgcca ataatttttc attttcttga
       61 caacttcttc tgaggggacg ttatcactct tccctctatt tttatcggat cttgtcaaca
      121 ctttattatc aatagaatca tctttgagaa aagactgggg cacgatggca tccacgtcgt
      181 agtcggagag ccgattgatg tccagttcct gatccacgta catgtccctg ccgttctgca
      241 ggtagtacag gtagagcttc tcattctgaa gctgggtgtt ttcaactggg tgttccttaa
      301 ggatttggga ccccagttct tttataccct cttcaatcct cttcatcctt tccctactgt
      361 tcttctgtcc cttctgggta gtttggttct ctcgggccat ctcgataacg atattctcgg
      421 gcttatgcct tcccattact ttgacgagtt catccacgac cttaacggtc tgcagtattc
      481 cctttttgat agctgggcta cctgcaagat tagcgatgtg ctcgtgaaga ctgtccccct
      541 ggccagaaac ttgtgctttc tggatgtcct ccttaaaggt gagagagtca tcatggatca
      601 actgcatgaa gttccggttg gcaaatccat cggacttaag aaaatccagg attgtctttc
      661 cactctgctt gtctcggatc ccattgatca gttttcttga cagccgcccc catcctgtat
      721 atcggcgcct cttgagctgt ttcatgactt tgtcgtcgaa gagatgagcg
//
