LOCUS       sequence-73494-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes human-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pCMV_dCas9_VP64
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..24)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcgactttg cgtttcttct tgggtgaccc tgaacccagt gagggcccgt cagccctgct
       61 gtctccaccg agctgagaga ggtcgattct tgtttcatag agccccgtaa ttgactgatg
      121 aatcagtgtg gcgtccagga cctcctttgt agaggtgtac cgctttctgt ctatggtggt
      181 gtcgaagtac ttgaaggctg caggcgcgcc caagttggtc agagtaaaca agtggataat
      241 gttttctgcc tgctccctga tgggcttatc cctgtgctta ttgtaagcag aaagcacctt
      301 atcgaggtta gcgtcggcga ggatcactct tttggagaat tcgcttattt gctcgatgat
      361 ctcatcaagg tagtgtttgt gttgttccac gaacagctgc ttctgctcat tatcttcggg
      421 agaccctttg agcttttcat agtggctggc cagatacaag aaattaacgt atttagaggg
      481 cagtgccagc tcgttacctt tctgcagctc gcccgcacta gcgagcattc gtttccggcc
      541 gttttcaagc tcaaagagag agtacttggg aagcttaatg atgaggtctt ttttgacctc
      601 tttatatcct ttcgcctcga gaaagtcgat ggggtttttt tcgaagcttg atcgctccat
      661 gattgtgatg cccagcagtt ccttgacgct tttgagtttt ttagacttcc ctttctccac
      721 tttggccaca accagtacac tgtaagcgac tgtaggagaa tcgaatccgc cgtatttctt
      781 ggggtcccaa tcttttttgc gtgcgatcag cttgtcgctg ttccttttcg ggaggatact
      841 t
//
