LOCUS       sequence-73500-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses MPMV TM fusion subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  TITLE     MPMV TM
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJexpress414-MPMV-TM
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      414..461
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(684..703)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(709..728)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        770..861
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctagaaata attttgttta acttttagga ggtaaaacat atggcgcacc accaccacca
       61 ccacagctgg gaaaacctgt acttccaagg cagctccacc ggtgcagcgg gtttgggcgt
      121 gagcattacc cagtatacca agctgagcca tcagctgatc agcgacgttc aggcgatttc
      181 tagcacgatc caagacctgc aagatcaggt cgactcgctg gcagaggttg tgctgcaaaa
      241 ccgtcgcggc ctggacctgt tgacggcgga acagggtggt atttgtctgg ccctgcagga
      301 gaaatgcagc ttttacgcta ataagagcgg tatcgtccgt gacaaaatca aaaatctgca
      361 agatgatttg gagcgccgtc gtcgccagct gattgataac taactcgagc cccctagcat
      421 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gcccctgaga cgcgtcaatc
      481 gagttcgtac ctaagggcga caccccataa ttagcccggg cgaaaggccc agtctttcga
      541 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg ggagtcccca
      601 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
      661 ccgcgctact gccgccaggc aaacaagggg tgttatgagc catattcagg tataaatggg
      721 ctcgcgataa tgttcagaat tggttaattg gttgtaacac tgacccctat ttgtttattt
      781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      841 taatattgaa aaaggaagaa tatgagtatt caacatttcc gtgtcgccct tattcccttt
      901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      961 gctgaagatc agttgggtgc acga
//
