LOCUS       sequence-73502-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX sequencing vector for subcloning, tetracycline marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pINIT_tet
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      527..554
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
       61 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
      121 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
      181 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
      241 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
      301 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
      361 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctgcatgaa
      421 gagcggccac cgaggccggg agcggatttg aacgttgcga agcaacggnc cggnnngggn
      481 gggngggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
      541 acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa
      601 tatgtatccg ctcatggagc taccggacag cggtgcggac tgttgtaact cagaataaga
      661 aatgaggccg ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc
      721 cactctctgt tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta
      781 cgaaacacgg aaaccgaa
//
