LOCUS       sequence-73513-e         304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized Cas9 nuclease from Streptococcus pyogenes 
            (Cbh-3X-FLAG-NLS-SpCas9-NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9 (PX165)
FEATURES             Location/Qualifiers
     source          1..304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
       61 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat
      121 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      181 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
      241 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
      301 gggg
//
