LOCUS       sequence-73515-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 with an N-terminal 
            3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is 
            synthetic, containing convenient restriction sites. Native hNKCC1 
            amino acid sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgagctctac aagggtgacc tggagccgag gcccacggct cctagctccg gcgcgccagg
       61 cctcgccgga gtcggcgaaa cgccgagcgc cgctgcgctg gccgcagcca gggtggaact
      121 gcccggaacc gctgtgccct cggtgccgga ggatgctgcg cccgcgagcc gggacggggg
      181 cggggtccgc gatgagggac cagccgccgc cggtgacggc ctgggcagac ccttggggcc
      241 taccccgagc cagagccgtt tccaggtgga cctcgtttcc gagaacgcag ggcgggctgc
      301 tgctgcggcg gcggcggctg ctgcggccgc cgccgctgct ggtgccgggg cgggggccaa
      361 gcagactcct gccgacgggg aagccagcgg cgagagcgag ccagctaaag gcagcgagga
      421 agccaaaggc cgcttccgcg ttaacttcgt agatcctgct gccagctcgt ccgcagaaga
      481 tagcctgtca gatgccgccg gggtcggagt cgatggaccc aacgtgagct tccagaacgg
      541 cggggacacg gtactgagcg agggcagcag cctccattcc ggtggcggcg gcggctccgg
      601 ccaccaccag cactactatt atgataccca caccaacacc tactatctgc gcaccttcgg
      661 ccacaacaca atggacgcgg tacccaggat cgaccactac cggcacacag ccgcgcagct
      721 cggcgagaag ctgctccggc ctagcctggc cgagctgcat gacgagctgg aaaaggaacc
      781 ttttgaggat ggctttgcaa atggggaaga aagcactcca acacgtgatg ctgtggtcac
      841 gtacacggcc gaaagtaaag gagtcgtgaa gtttggctgg atcaagggtg tacta
//
