LOCUS       sequence-73516-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus
            and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is 
            synthetic, containing convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutati...
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agggtgacct ggagccgagg cccacggctc ctagctccgg cgcgccaggc ctcgccggag
       61 tcggcgaaac gccgagcgcc gctgcgctgg ccgcagccag ggtggaactg cccggaaccg
      121 ctgtgccctc ggtgccggag gatgctgcgc ccgcgagccg ggacgggggc ggggtccgcg
      181 atgagggacc agccgccgcc ggtgacggcc tgggcagacc cttggggcct accccgagcc
      241 agagccgttt ccaggtggac ctcgtttccg agaacgcagg gcgggctgct gctgcggcgg
      301 cggcggctgc tgcggccgcc gccgctgctg gtgccggggc gggggccaag cagactcctg
      361 ccgacgggga agccagcggc gagagcgagc cagctaaagg cagcgaggaa gccaaaggcc
      421 gcttccgcgt taacttcgta gatcctgctg ccagcaattc cgcagaagat cagctgtcag
      481 atgccgccgg ggtcggagtc gatggaccca acgtgcagtt ccagaacggc ggggacgctg
      541 tactgagcga gggccgtagc ctccattccg gtggcggcgg cggcgagggc caccaccagc
      601 actactatta tgatgctcac gctaaccagt actatctgcg cgctttcggc cacaacgcta
      661 tggacgcggt acccaggatc gaccactacc ggcacgctgc cgcgcagctc ggcgagaagc
      721 tgctccggcc taatctggcc gagctgcatg acgagctgga aaaggaacct tttgaggatg
      781 gctttgcaaa tggggaagaa tctgctccac agcgtgatgc tgtggtcgag taccgtgccg
      841 aaagtaaagg agtcgtgaag tttggctgga tcaagggtgt gctagtacgt tgtatgttaa
      901 acatttgggg
//
