LOCUS       sequence-73520-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant with HA tag inserted into extracellular
            loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is 
            synthetic, containing convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agggtgacct ggagccgagg cccacggctc ctagctccgg cgcgccaggc ctcgccggag
       61 tcggcgaaac gccgagcgcc gctgcgctgg ccgcagccag ggtggaactg cccggaaccg
      121 ctgtgccctc ggtgccggag gatgctgcgc ccgcgagccg ggacgggggc ggggtccgcg
      181 atgagggacc agccgccgcc ggtgacggcc tgggcagacc cttggggcct accccgagcc
      241 agagccgttt ccaggtggac ctcgtttccg agaacgcagg gcgggctgct gctgcggcgg
      301 cggcggctgc tgcggccgcc gccgctgctg gtgccggggc gggggccaag cagactcctg
      361 ccgacgggga agccagcggc gagagcgagc cagctaaagg cagcgaggaa gccaaaggcc
      421 gcttccgcgt taacttcgta gatcctgctg ccagctcgtc cgcagaagat agcctgtcag
      481 atgccgccgg ggtcggagtc gatggaccca acgtgagctt ccagaacggc ggggacacgg
      541 tactgagcga gggcagcagc ctccattccg gtggcggcgg cggctccggc caccaccagc
      601 actactatta tgatacccac accaacacct actatctgcg caccttcggc cacaacacaa
      661 tggacgcggt acccaggatc gaccactacc ggcacacagc cgcgcagctc ggcgagaagc
      721 tgctccggcc tagcctggcc gagctgcatg acgagctgga aaaggaacct tttgaggatg
      781 gctttgcaaa tggggaagaa agcactccaa cacgtgatgc tgtggtcacg tacacggccg
      841 aaagtaaagg agtcgtgaag tttggctgga tca
//
