LOCUS       sequence-73525-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 truncation mutant lacking extracellular loop 
            #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian 
            cells. cDNA is synthetic, containing convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctctacaagg gtgacctgga gccgaggccc acggctccta gctccggcgc gccaggcctc
       61 gccggagtcg gcgaaacgcc gagcgccgct gcgctggccg cagccagggt ggaactgccc
      121 ggaaccgctg tgccctcggt gccggaggat gctgcgcccg cgagccggga cgggggcggg
      181 gtccgcgatg agggaccagc cgccgccggt gacggcctgg gcagaccctt ggggcctacc
      241 ccgagccaga gccgtttcca ggtggacctc gtttccgaga acgcagggcg ggctgctgct
      301 gcggcggcgg cggctgctgc ggccgccgcc gctgctggtg ccggggcggg ggccaagcag
      361 actcctgccg acggggaagc cagcggcgag agcgagccag ctaaaggcag cgaggaagcc
      421 aaaggccgct tccgcgttaa cttcgtagat cctgctgcca gctcgtccgc agaagatagc
      481 ctgtcagatg ccgccggggt cggagtcgat ggacccaacg tgagcttcca gaacggcggg
      541 gacacggtac tgagcgaggg cagcagcctc cattccggtg gcggcggcgg ctccggccac
      601 caccagcact actattatga tacccacacc aacacctact atctgcgcac cttcggccac
      661 aacacaatgg acgcggtacc caggatcgac cactaccggc acacagccgc gcagctcggc
      721 gagaagctgc tccggcctag cctggccgag ctgcatgacg agctggaaaa ggaacctttt
      781 gaggatggct ttgcaaatgg ggaagaaagc actccaacac gtgatgctgt ggtcacgtac
      841 acggccgaaa gtaaaggagt cgtgaagttt ggctggatca
//
