LOCUS       sequence-73526-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 C630A (NT399)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgagctcta caagggtgac ctggagccga ggcccacggc tcctagctcc ggcgcgccag
       61 gcctcgccgg agtcggcgaa acgccgagcg ccgctgcgct ggccgcagcc agggtggaac
      121 tgcccggaac cgctgtgccc tcggtgccgg aggatgctgc gcccgcgagc cgggacgggg
      181 gcggggtccg cgatgaggga ccagccgccg ccggtgacgg cctgggcaga cccttggggc
      241 ctaccccgag ccagagccgt ttccaggtgg acctcgtttc cgagaacgca gggcgggctg
      301 ctgctgcggc ggcggcggct gctgcggccg ccgccgctgc tggtgccggg gcgggggcca
      361 agcagactcc tgccgacggg gaagccagcg gcgagagcga gccagctaaa ggcagcgagg
      421 aagccaaagg ccgcttccgc gttaacttcg tagatcctgc tgccagctcg tccgcagaag
      481 atagcctgtc agatgccgcc ggggtcggag tcgatggacc caacgtgagc ttccagaacg
      541 gcggggacac ggtactgagc gagggcagca gcctccattc cggtggcggc ggcggctccg
      601 gccaccacca gcactactat tatgataccc acaccaacac ctactatctg cgcaccttcg
      661 gccacaacac aatggacgcg gtacccagga tcgaccacta ccggcacaca gccgcgcagc
      721 tcggcgagaa gctgctccgg cctagcctgg ccgagctgca tgacgagctg gaaaaggaac
      781 cttttgagga tggctttgca aatggggaag aaagcactcc aacacgtgat gctgtggtca
      841 cgtacacggc cgaaagtaaa ggagtcgtga agtttggctg gatca
//
