LOCUS       sequence-73538-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid with mRuby2 for Golden Gate reaction of TALE 
            assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM4
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..829
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gggattgccg cacgcgccgg aattgatcag aagagtcaat cgccgtattg gcgaacgcac
       61 gtcccatcgc gttgcgggat ccactggagg cggaggtggc tccggcaccg cctcctccat
      121 ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg
      181 ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg
      241 aactcaaacc atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat
      301 tcttgccacg tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc
      361 tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga
      421 agatggtgga gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta
      481 ccacgtccaa gtcagagggg taaactttcc ctccaatggt cccgtgatgc agaagaagac
      541 caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata
      601 cactcatatg gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac
      661 ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca
      721 ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc
      781 agttgccaag ttcgccgggc ttggtggtgg gatggacgag ctgtacaag
//
