LOCUS       sequence-73541-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant lacking cysteine residues within 
            intracellular loops and with an N-terminal 3xFLAG-YFP tag in 
            mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 intracellular-cystein...
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttatgaatag aaggtaagga cactctgatg attcccacga actagtagga ttggtggtag
       61 atccttagat agtgcttcta accatgccat gtagagagca ctagaaacgg caccctttcg
      121 tgcgaccggt aaactcatca caataatgtt agctgtgctt gaatgttcct ttaataactc
      181 atttaacctg atctgccggt atgtcttggt cttatacagc tccaattcat tatctgttat
      241 tcgccagggt tcatcttctt tcattttatc tgcgatatct tgctctttat catcttcgtg
      301 aagtctgtat ggctcaatga tttcctcgaa agctataatg ttttctttct ttggtttggt
      361 attgatgtct cctagaacca tgatgtcaga aaaatcgatt ctgaacttgc tcagtaaagt
      421 agccatcgct cttctgtcat ggtctattct gtttatcttt ccaccaatga atactctgat
      481 cttagagtct ttccattttt tcttggtcgt cagaaggtaa ggtatgagta atgtgagacc
      541 tccgtcatca aaaagccacc agacatcaat agtattcttt ccttgttttt tctgaaactg
      601 tgtactagct tcaagaagct tttggtcagc tacattcaaa ggcacaatag ggcctttgga
      661 ttcttttttc aacagtggtt gagttgcagt cttgccatct tcttcctcga ctttatgggt
      721 aattggtttt tcactgaggg gtttcgaagt atctaaatcg gactttttac tatattccac
      781 acttactacg acatccttgg tgccaggaga tttctcttgt gatgacaata attcttcttg
      841 tccctgcagg tgactaatat ccagaccttc ttttaggcga ataaccacta cgccatattg
      901 tatgtcaaaa gcatcgtgaa ataagtttat gtacatatcc acatccctca tatctgcttg
      961 caaccaatct ttcttaaatc caagga
//
