LOCUS       sequence-73550-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant lacking cysteine residues within 
            transmembrane domains and with an N-terminal 3xFLAG-YFP tag in 
            mammalian cells. cDNA is synthetic,containing convenient restriction
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attatgaata gaaggtaagg acactctgat gattcccacg aactagtagg attggtggta
       61 gatccttaga tagtgcttct aaccatgcca tgtagagagc actagaaacg gcaccctttc
      121 gtgcgaccgg taaactcatc acaataatgt tagctgtgct tgaatgttcc tttaataact
      181 catttaacct gatctgccgg tatgtcttgg tcttatacag ctccaattca ttatctgtta
      241 ttcgccaggg ttcatcttct ttcattttat ctgcgatatc ttgctcttta tcatcttcgt
      301 gaagtctgta tggctcaatg atttcctcga aagctataat gttttctttc tttggtttgg
      361 tattgatgtc tcctagaacc atgatgtcag aaaaatcgat tctgaacttg ctcagtaaag
      421 tagccatcgc tctccggtca tggtctattc tgtttatctt tccaccaatg aatactctga
      481 tcttacagtc tttccatttt ttcttggtcg tcagaaggta aggtatcaat aaggtcagac
      541 ctccgtcatc aaaaagccac cagacatcaa tagtattctt tccttgtttt ttctgaaact
      601 gtgtactagc ttcaagaagc ttttggtcag ctacattcaa aggcacaata gggcctttgg
      661 attctttttt caacagtggt tgagttgcag tcttgccatc ttcttcctcg actttatggg
      721 taattggttt ttcactgagg ggtttcgaag tatctaaatc ggacttttta ctatattcca
      781 cacttactac gacatccttg gtgccaggag atttctcttg tgatgacaat aattcttctt
      841 gtccctgcag gtgactaata tccagacctt cttttaggcg aataaccact acgccatatt
      901 gtatgtcaaa agcatcgtga aataagttta tgtacatatc cacatccctc atatctgctt
      961 gcaaccaatc tttcttaaat cc
//
