LOCUS       sequence-73553-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 C820S (NT573)
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tattatgaat agaaggtaag gacactctga tgattcccac gaactagtag gattggtggt
       61 agatccttag atagtgcttc taaccatgcc atgtagagag cactagaaac ggcacccttt
      121 cgtgcgaccg gtaaactcat cacaataatg ttagctgtgc ttgaatgttc ctttaataac
      181 tcatttaacc tgatctgccg gtatgtcttg gtcttataca gctccaattc attatctgtt
      241 attcgccagg gttcatcttc tttcatttta tctgcgatat cttgctcttt atcatcttcg
      301 tgaagtctgt atggctcaat gatttcctcg aaagctataa tgttttcttt ctttggtttg
      361 gtattgatgt ctcctagaac catgatgtca gaaaaatcga ttctgaactt gctcagtaaa
      421 gtagccatcg ctctccggtc atggtctatt ctgtttatct ttccaccaat gaatactctg
      481 atcttacagt ctttccattt tttcttggtc gtcagaaggt aaggtatcaa taaggtcaga
      541 cctccgtcat caaaaagcca ccagacatca atagtattct ttccttgttt tttctgaaac
      601 tgtgtactag cttcaagaag cttttggtca gctacattca aaggcacaat agggcctttg
      661 gattcttttt tcaacagtgg ttgagttgca gtcttgccat cttcttcctc gactttatgg
      721 gtaattggtt tttcactgag gggtttcgaa gtatctaaat cggacttttt actatattcc
      781 acacttacta cgacatcctt ggtgccagga gatttctctt gtgatgacaa taattcttct
      841 tgtccctgca ggtgactaat atccagacct tcttttaggc gaataaccac tacgccatat
      901 tgtatgtcaa aagcatcgtg aaataagttt atgtacatat ccacatccct catatctgct
      961 tgcaaccaat ctttcttaaa tccaagga
//
