LOCUS       sequence-73555-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 C630S mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 C630S (NT400)
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttattatgaa tagaaggtaa ggacactctg atgattccca cgaactagta ggattggtgg
       61 tagatcctta gatagtgctt ctaaccatgc catgtagaga gcactagaaa cggcaccctt
      121 tcgtgcgacc ggtaaactca tcacaataat gttagctgtg cttgaatgtt cctttaataa
      181 ctcatttaac ctgatctgcc ggtatgtctt ggtcttatac agctccaatt cattatctgt
      241 tattcgccag ggttcatctt ctttcatttt atctgcgata tcttgctctt tatcatcttc
      301 gtgaagtctg tatggctcaa tgatttcctc gaaagctata atgttttctt tctttggttt
      361 ggtattgatg tctcctagaa ccatgatgtc agaaaaatcg attctgaact tgctcagtaa
      421 agtagccatc gctctccggt catggtctat tctgtttatc tttccaccaa tgaatactct
      481 gatcttacag tctttccatt ttttcttggt cgtcagaagg taaggtatca ataaggtcag
      541 acctccgtca tcaaaaagcc accagacatc aatagtattc tttccttgtt ttttctgaaa
      601 ctgtgtacta gcttcaagaa gcttttggtc agctacattc aaaggcacaa tagggccttt
      661 ggattctttt ttcaacagtg gttgagttgc agtcttgcca tcttcttcct cgactttatg
      721 ggtaattggt ttttcactga ggggtttcga agtatctaaa tcggactttt tactatattc
      781 cacacttact acgacatcct tggtgccagg agatttctct tgtgatgaca ataattcttc
      841 ttgtccctgc aggtgactaa tatccagacc ttcttttagg cgaataacca ctacgccata
      901 ttgtatgtca aaagcatcgt gaaataagtt tatgtacata tccacatccc tcatatctgc
      961 ttgcaacc
//
