LOCUS       sequence-73567-e        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Mff fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz 
            GK
  TITLE     ER tubules mark sites of mitochondrial division.
  JOURNAL   Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385.
            Epub 2011 Sep 1.
  PUBMED    21885730
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1207385"; journalName: "Science"; date: 
            "2011-10-21- 21"; volume: "334"; issue: "6054"; pages: "358-62"
COMMENT     Sequence Label: GFP-Mff
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..777
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
       61 atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat
      121 ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac
      181 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc
      241 ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag
      301 cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc
      361 ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg
      421 gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat
      481 aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat
      541 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc
      601 gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac
      661 tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc
      721 tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtcc
      781 ggactcagat ctcgagtgat gagtaaagga acaagcagtg acacatcact aggaagggtg
      841 agcagggcag catttccttc tcccactgct gctgagatgg cagaaattag tcgaattcag
      901 tacgaaatgg aatatactga aggcattagt cagcgaatga gggtcccaga aaagttaaaa
      961 gtagcaccgc caaacgctga cctggaacaa ggattccaag aaggagttcc aaatgctagt
     1021 gtgataatgc aagttccgga gaggattgtt gtagcaggaa ataatgaaga tgtttcattt
     1081 tca
//
