LOCUS       sequence-7358-ex        4178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Trame CB, McKay DB
  TITLE     Structure of Haemophilus influenzae HslU protein in crystals with 
            one-dimensional disorder twinning.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1079-90.
  PUBMED    11468391
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1079-90."
COMMENT     Sequence Label: HI.HslU.RSETB.fl.wt
FEATURES             Location/Qualifiers
     source          1..4178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1466..1484)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1480..1527
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1624..2079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1711..1730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1921..1942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2160..2264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2265..2333,2334..3125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRPPRYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3296..3884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3785..3804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4038..4055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4140..4159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4140..4158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacat atgtctgaaa tgactcctcg
       61 cgaaatagtt tctgaattag atcaacatat tattggtcaa gccgatgcaa aacgtgctgt
      121 ggcgattgcg ttgcgtaacc gttggcgtcg tatgcagctt caagaacctt tgcgtcatga
      181 agtaactcca aaaaatattt taatgattgg cccaactggt gtgggtaaaa ctgaaattgc
      241 gcgccgttta gcaaaattag ctaacgcacc atttatcaaa gtggaagcaa cgaaatttac
      301 tgaagtaggt tacgtgggga aagaggtgga ttccattatt cgtgatttaa cggatagtgc
      361 gatgaaactt gttcgccaac aagaaattgc gaagaacaga gcaagagctg aagacgtggc
      421 tgaagagcgt attttggatg ctttacttcc tccagcaaaa aatcagtggg gcgaagttga
      481 aaaccatgat agtcacagca gtactcgcca agcgttccgt aaaaaattac gtgaaggtca
      541 attagatgat aaagaaatcg aaattgatgt gtctgctggc gtatctatgg gggttgaaat
      601 tatggctcct ccaggcatgg aagaaatgac aaatcaactg caatcattgt tccaaaattt
      661 gggttcagac aaaacgaaaa aacgcaaaat gaaaattaaa gatgcgttga aagcgttgat
      721 tgatgatgaa gcagcgaaat taattaaccc tgaagaactt aaacaaaaag ccattgatgc
      781 tgtggaacaa aatggtatcg tctttatcga tgaaatcgac aaaatttgta agaaaggtga
      841 atacagtggt gctgatgtat cgcgtgaagg tgtgcaacgt gatttacttc cgttagtaga
      901 aggctcgaca gttagcacca agcacggaat ggttaaaact gatcatatct tatttattgc
      961 atctggtgca ttccaagttg ctcgcccttc agatttaatt cctgaactac aagggcgttt
     1021 gccgattcgt gttgaactta ccgcattaag tgcggcggat tttgagcgta ttttaacaga
     1081 gccacatgct tctttaaccg aacaatataa agcgttgatg gcaacagaag gggtgaatat
     1141 tgcattcaca acagatgcag tgaagaaaat tgctgaggcg gcattccgag tgaatgaaaa
     1201 aacggaaaat atcggtgcac gccgtttgca tacggtaatg gagcgtttga tggataaaat
     1261 ttctttcagc gcaagcgaca tgaatggtca aacggtcaat attgatgcag cttatgttgc
     1321 ggatgcactt ggcgaagtgg ttgaaaatga agatttgagc cgttttattc tttaaagata
     1381 aaaattttac gggtaccgag ctcgaattcg aagcttgatc cggctgctaa caaagcccga
     1441 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     1501 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atctggcgta
     1561 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1621 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1801 ttagagcttt acggcacctc gaccgcaaaa aacttgattt gggtgatggt tcacgtagtg
     1861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1921 gtggactctt gttccaaact ggaacaacac tcaaccctat cgcggtctat tcttttgatt
     1981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaatat
     2041 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc gcctgatgcg gtattttctc
     2101 cttacgcatc tgtgcggtat ttcacaccgc atacaggtgg cacttttcgg ggaaatgtgc
     2161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2461 tgatgagcac ttttaaagtt ctgctatgtg atacactatt atcccgtatt gacgccgggc
     2521 aagagcaact cggtcgcccc ccgcggtatt ctcagaatga cttggttgag tactcaccag
     2581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2761 agctgaatga agccatacca aacgacgaga gtgacaccac gatgcctgta gcaatgccaa
     2821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2881 tagactgaat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3001 cactggggcc agatggtaag cgctcccgta tcgtagttat ctacacgacg gggagtcagg
     3061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3421 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3901 cctttttacg gttcctgggc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3961 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4021 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4081 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gatctcgatc ccgcgaaatt
     4141 aatacgactc actataggga gaccacaacg gtttccct
//
