LOCUS       sequence-73581-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway adapted transfer of Neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Xu D, Brandan CP, Basombrio MA, Tarleton RL
  TITLE     Evaluation of high efficiency gene knockout strategies for 
            Trypanosoma cruzi.
  JOURNAL   BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90.
  PUBMED    19432966
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2009-05-11"; volume: "9:90. doi"; pages: " 
            10.1186/1471-2180-9-90"
COMMENT     Sequence Label: pDONR221-Neo
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
       61 ctatcgcctt cttgacgagt tcttctgagg ggatcgatcc gtgcacaggc gttagccact
      121 ctcaggattg ccagcaagtc ttctgaattt gatctttgga tgctcgggca gctcaaagag
      181 gaatcttagt cacaacctgc cgaaggagcg ccgcttcttc aagattgtgt cgtggtacga
      241 caacgagtgg ggatactccc accgcgtggt ggaccttgta cgccacatgg cctcgaagga
      301 tcgttcggca aggttgtagg cgtggcgatg acttcaggtc tttcttttgc gaatagggat
      361 cttataatac acgatgcgtg tcccgtgatg atcgttaccg gtgctgccac gatccaagtg
      421 acacagtgtc aagagcaaaa caattttact tttcccttta aggacaacaa caaaaaaata
      481 tataactttt ttttcttttt ttttttgaaa ttatatttat ggtcatcttt gggaaacaaa
      541 aagcagcaat ttaatgatgc ggaaggatga gtgaaataat gtttaatcaa tgtacgagga
      601 tttggggtat tgcaaggaaa atgtagatga tttgattggg tgtatgatgc agcttgtggt
      661 aattttttac aactttgcgt tattggaaga ttttggttgt tcagtttctt tttgtttata
      721 actgactgtt ttaatttagt gtgatttttt ttttttaagg atttaagctg tttttctttt
      781 tttttctttt tcttcactga tggtgtttaa ggtgtagttt agtgtttgtg cattatgcgt
      841 cgta
//
