LOCUS       sequence-73582-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian S. thermophilus #1 Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-ST1cas
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccacaagc agctggccac caagatccgc ctgtggcacc agcagggcga gcgctgcctg
       61 tacaccggca agaccatcag catccacgac ctgatcaaca acagcaacca gttcgaggtg
      121 gaccacatcc tgcccctgag catcaccttc gacgacagcc tggccaacaa ggtgctggtg
      181 tacgccaccg ccaaccagga gaagggccag cgcaccccct accaggccct ggacagcatg
      241 gacgacgcct ggagcttccg cgagctgaag gccttcgtgc gcgagagcaa gaccctgagc
      301 aacaagaaga aggagtacct gctgaccgag gaggacatca gcaagttcga cgtgcgcaag
      361 aagttcatcg agcgcaacct ggtggacacc ctctacgcca gccgcgtggt gctgaacgcc
      421 ctgcaggagc acttccgcgc ccacaagatc gacaccaagg tgagcgtggt gcgcggccag
      481 ttcaccagcc agctgcgccg ccactggggc atcgagaaga cccgcgacac ctaccaccac
      541 cacgccgtgg acgccctgat cattgcggct tctagccagc tgaacctgtg gaagaagcag
      601 aagaacaccc tggtgagcta cagcgaggac cagctgctgg acatcgagac cggcgagctg
      661 atcagcgacg acgagtacaa ggagagcgtg ttcaaggccc cctaccagca cttcgtggac
      721 accctgaaga gcaaggagtt cgaggacagc atcctgttca gctaccaggt ggacagcaag
      781 ttcaaccgca agatcagcga cgccaccatc tacgccaccc gccaggccaa ggtgggcaag
      841 gacaaggccg acgagaccta cgtgctgggc aagatcaagg acatctacac ccaggacggc
      901 tacgacgcct tcatgaagat ctacaagaa
//
