LOCUS       sequence-73585-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-NMcas
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggaccgcgag aaggccgccg ccaagttccg cgagtacttc cccaacttcg tgggcgagcc
       61 caagagcaag gacatcctga agctgcgcct gtacgagcag cagcacggca agtgcctgta
      121 cagcggcaag gagatcaacc tgggccgcct gaacgagaag ggctacgtgg agatcgacca
      181 cgccctgccc ttcagccgca cctgggacga cagcttcaac aacaaggtgc tggtgctggg
      241 cagcgagaac cagaacaagg gcaaccagac cccctacgag tacttcaacg gcaaggacaa
      301 cagccgcgag tggcaggagt tcaaggcccg cgtggagacc agccgcttcc cccgcagcaa
      361 gaagcagcgc atcctgctgc agaagttcga cgaggacggc ttcaaggagc gcaacctgaa
      421 cgacacccgc tacgtgaacc gcttcctgtg ccagttcgtg gccgaccgca tgcgcctgac
      481 cggcaagggc aagaagcgcg tgttcgccag caacggccag atcaccaacc tgctgcgcgg
      541 cttctggggc ctgcgcaagg tgcgcgccga gaacgaccgc caccacgccc tggacgccgt
      601 ggtggtggcc tgcagcaccg tggccatgca gcagaagatc acccgcttcg tgcgctacaa
      661 ggagatgaac gccttcgacg gtaaaaccat cgacaaggag accggcgagg tgctgcacca
      721 gaagacccac ttcccccagc cctgggagtt cttcgcccag gaggtgatga tccgcgtgtt
      781 cggcaagccc gacggcaagc ccgagttcga ggaggccgac acccccgaga agctgcgcac
      841 cctgctggcc gagaagctga gcagccgccc tgaggccgtg cacgagtacg tgactcctct
      901 gttcgtgagc cgcgccccca accgcaagat gagcggtcag
//
