LOCUS       sequence-73586-e         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway adapted transfer of Phleomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Xu D, Brandan CP, Basombrio MA, Tarleton RL
  TITLE     Evaluation of high efficiency gene knockout strategies for 
            Trypanosoma cruzi.
  JOURNAL   BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90.
  PUBMED    19432966
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2009-05-11"; volume: "9:90. doi"; pages: " 
            10.1186/1471-2180-9-90"
COMMENT     Sequence Label: pDONR221-Phelo
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(102..476)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQY"
     protein_bind    complement(480..579)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
       61 tttcttataa tgccaacttt gtacaagaaa gctgggttcg gtcagtactg ctcctcggcc
      121 acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc
      181 tcgccgatct cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc
      241 gaccactcgg cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc
      301 ggcaccacct ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg
      361 gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc
      421 gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggta
      481 gcctgctttt ttgtacaaag ttggcattat aaaaaagcat tgctcatcaa tttgttgcaa
      541 cgaacaggtc actatcagtc aaaataaaat cattatttgg ggccc
//
