LOCUS       sequence-73595-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creates a 3' Fusion of EGFP to insert for expression in Xenopus 
            oocytes or mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     EGFP Fuxion pXOON plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3' EGFP pXOON
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           102..144
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             join(227..229,230..232,233..745)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(272..293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(533..552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     880..901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagagg tacccagctt gcttgttctt tttgcagaag
      121 ctcagaataa acgctcaact ttggcagatc aattccccgg ggatccgaat tcgtgagaaa
      181 gcttaccggt gatatcggcc ccgcgggcca ccgatcgggc ggccgcatgg tgagcaaggg
      241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      421 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      601 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      661 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      721 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      781 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      841 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      901 gaccgccgcc gggatcactc tcggcatgg
//
