LOCUS       sequence-73604-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  visualization of the ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz 
            GK
  TITLE     ER tubules mark sites of mitochondrial division.
  JOURNAL   Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385.
            Epub 2011 Sep 1.
  PUBMED    21885730
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1207385"; journalName: "Science"; date: 
            "2011-10-21- 21"; volume: "334"; issue: "6054"; pages: "358-62"
COMMENT     Sequence Label: BFP-KDEL
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(183..878)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     promoter        complement(961..1012)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(990..1010)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatca gttatctaga tccggtggat cccgggcccg cggtacctca tagctcgtct
      181 ttattaagct tgtgccccag tttgctaggg aggtcgcagt atctggccac tgccacctcg
      241 tgctgctcga cgtaggtctc gttgttggcc tccttgattc tttccagtct gtagtccaca
      301 tagtagacgc caggcatctt gaggttctta gcgggtttct tggatctata tgtggtcttg
      361 atgtttgcga tcagatggct cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc
      421 aggccgccgt cagcggggta cagcgtctcg gtgaaggcct cccagccgag tgttttcttc
      481 tgcatcacag ggccgttgga tgtgaagttc acccctctga tcttgacgtt gtagatgagg
      541 cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg
      601 gtgactctct cccatgtgaa gccctcaggg aaggactgct tgaagaagtc ggggatgccc
      661 tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc tagtagccag gatgtcgaag
      721 gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt gccctcgtag
      781 ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt tgtccacggt gccctccatg
      841 tacagcttca tgtgcatgtt ctccttaatc agctcgctgg cccgcgccgc gctgagcagc
      901 agcagcatcg cggccaccag ggagagcttc atgctagcgg atctgacggt tcactaaacc
      961 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     1021 ggagttgt
//
