LOCUS       sequence-73605-e        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  visualization of the ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz 
            GK
  TITLE     ER tubules mark sites of mitochondrial division.
  JOURNAL   Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385.
            Epub 2011 Sep 1.
  PUBMED    21885730
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1207385"; journalName: "Science"; date: 
            "2011-10-21- 21"; volume: "334"; issue: "6054"; pages: "358-62"
COMMENT     Sequence Label: BFP-KDEL
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             99..794
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     polyA_signal    954..1035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(991..1010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1045..1064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatgaag ctctccctgg
       61 tggccgcgat gctgctgctg ctcagcgcgg cgcgggccag cgagctgatt aaggagaaca
      121 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
      181 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
      241 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
      301 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
      361 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
      421 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
      481 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
      541 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
      601 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
      661 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
      721 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
      781 ggcacaagct taataaagac gagctatgag gtaccgcggg cccgggatcc accggatcta
      841 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      901 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta t
//
