LOCUS       sequence-73607-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  initial cloning vector for mLIP fragment of C/EBPbeta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  TITLE     Jacob Freidman lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPCR-Script-mLIP
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      527..536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(594..614)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(594..612)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(633..649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(633..649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(646..668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    657..673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(681..687,688..705,706..711))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccgggggat ccgcccgcgc gatatcctag cagtggcccg ccgaggccag
      121 cagcggctcg ggcagctgct tgaacaagtt ccgcagggtg ctgagctctc gcgacagctg
      181 ctccaccttc ttctgcagcc gctcgttctc cgccgtcagc tccagcacct tgtgctgcgt
      241 ctccaggttg cgcatcttgg ccttgtcgcg gctcttgcgc accgcgatgt tgttgcgctc
      301 gcgccgcatc ttgtactcgt cgctcagctt gtccaccgtc ttcttggcct tggccttggc
      361 gggcgcggcc ggcggccccg cgaagcaggc ggcgggcgcg gccttggcgt cggcggggct
      421 cggcgtgccg ggcgggctgg acgacgacga cgtggacagg ctgccgctgc tgccgctcgg
      481 cgtcgcctgg tagcccaggt aggcgcgcag ggcgaacggg aaaccggccg ccatggtggg
      541 gtaccgcgtg ggctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
      601 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      661 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcct
//
