LOCUS       sequence-73613-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral vector encoding for GFP and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Zhao C, Teng EM, Summers RG, Ming GL, Gage FH
  TITLE     Distinct morphological stages of dentate granule neuron maturation 
            in the adult mouse hippocampus.
  JOURNAL   J Neurosci. 2006 Jan 4. 26(1):3-11.
  PUBMED    16399667
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2006 Jan 4. 26(1):3-11."
COMMENT     Sequence Label: CAG-GFP-IRES-CRE
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(26..600,601..603,604..612)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(193..210)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     420..439
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gagctgtaca agtaaagcgg ccgcgcccct ctccctcccc cccccctaac gttactggcc
       61 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
      121 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
      181 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
      241 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
      301 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
      361 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
      421 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
      481 tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa
      541 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
      601 atggccacaa ccatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc
      661 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg
      721 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc
      781 aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta
      841 tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac
      901 atgcttcatc gtcggtcc
//
