LOCUS       sequence-73615-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector contains CAG promoter , CMV enhancer and a large 
            synthetic intron that used to allow the ubiquitous expression of 
            GFP-fused Cre recombinase .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Tashiro A, Zhao C, Gage FH
  TITLE     Retrovirus-mediated single-cell gene knockout technique in adult 
            newborn neurons in vivo.
  JOURNAL   Nat Protoc. 2006;1(6):3049-55.
  PUBMED    17406567
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(6)"; pages: "3049-55"
COMMENT     Sequence Label: CAG-GFP/cre
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..48
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctgtaca agtccggaga gcaaaagctg atttctgagg aggatctggg aggacccaag
       61 aagaagagga aggtgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc
      121 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg
      181 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc
      241 aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta
      301 tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac
      361 atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt
      421 atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg
      481 ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag
      541 gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa
      601 attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat
      661 attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg
      721 gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac
      781 tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta
      841 tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag
      901 gatgactctg gtca
//
