LOCUS       sequence-73622-e        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Sec61 beta fused to mCherry, used as a general ER 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Zurek N, Sparks L, Voeltz G
  TITLE     Reticulon short hairpin transmembrane domains are used to shape ER 
            tubules.
  JOURNAL   Traffic. 2011 Jan;12(1):28-41. doi: 
            10.1111/j.1600-0854.2010.01134.x. Epub 2010 Nov 12.
  PUBMED    20955502
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01134.x"; journalName: "Traffic"; date: 
            "2011-01"; volume: "12"; issue: "1"; pages: "28-41"
COMMENT     Sequence Label: mCh-Sec61 beta
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(145..864)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctcactt gtacagctca tccatgccgt gggtgatggc
      181 ggcggcggtc acgaagccga agtagatcat gtgatcgcgc ttctcgttgg ggtccttgga
      241 cagggcgctc tgggtggaca ggtagtggtt atcgggcagc agcacagggc catcgccgat
      301 gggggtattc tgctggtagt ggtcggccag ctgcacgctg ccatcctcga tgttgtggcg
      361 gatcttgaag ttcaccttga tgccattctt ggccttgtcg gtcatgatgt acacattgtg
      421 ggcgttgtag ttgtactcca tcttattgcc caggatgttg ccatcctcct tgaaatcggt
      481 gccggtcagc tcgatgcgat tcaccagggt atcgccctcg aacttcacct cggcgcgcga
      541 cttgtagttg ccgtcatcct cgaagaagat ggtgcgctcc tggatgtagc cctcaggcat
      601 ggcgctcttg aagaagtcgt gctgcttcat gtgatcgggg tagcgtgaga agcactgcac
      661 gccgtagctc agggtggtca ccagggtggg ccagggcaca ggcagcttgc cggtggtgca
      721 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cgctcacgct
      781 gaacttgtgg ccattcacat cgccattcag ctcgatcagg atgggcacga tgccggtgaa
      841 cagctcggcg cccttgctca ccatgaccgg tggatcccgg gcccgcggta ccgtcgactg
      901 cagaattcct acgaacgagt gtacttgccc caaatgtgca acataaatac agaagcgatg
      961 aacagaagac tcataaccaa tactggaaca gggccaactt tgagcccagg tgaatcttct
     1021 gtgtagaatc gccacatccc cccggtgcct gccgaggttg tgcggcctgc actccttgtc
     1081 ccacagctgg catttttcct ctgc
//
