LOCUS       sequence-73643-e       10768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305590110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-9- 9"; volume: "110"; issue: "28"; pages: "11284-9"
COMMENT     Sequence Label: pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..10768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..786
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(115..136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(376..395)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     723..744
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(822..1396,1397..1399,1400..1408)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(989..1006)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1216..1235
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1417..1426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1423..2133
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1568..1586)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1836..1855
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2146..2734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2199..2219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2617..2628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2879..2912)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2932..3112
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3137..3155)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3138..3155)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3445..3464)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3678..3697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3797..3819
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3857..3875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3943..4047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4048..4116,4117..4908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4266..4285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5079..5667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5568..5587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5821..5838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5908..5928)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5913..6242
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6093..6228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7376..7441
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7571..7591
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8016..8150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8053..8072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8107..8126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8319..8952
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8999..9124
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9621..9854
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10039..10083
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10350..10467
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10523..10734
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tcgagtaact aactaaacca tgggttgata aaccatgggt tgataaacca tgggttgatt
       61 accatgggcg aattcagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      181 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      241 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      301 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      361 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      541 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      661 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
      721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      781 tacaagttcg aataagcatg catctagggc ggccaattcc gcccctctcc ctcccccccc
      841 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtga
      901 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
      961 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     1021 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     1081 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     1141 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     1201 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     1261 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     1321 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     1381 aaaacacgat gataagcttg ccacaacccg ggatccgcca ccatggtgag caagggcgag
     1441 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1501 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1561 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     1621 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1681 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1741 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     1801 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1861 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     1921 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1981 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     2041 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2101 tccaccggcg gcatggacga gctgtacaag taagcggccg ccgataatca acctctggat
     2161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2521 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     2581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     2641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     2701 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg caattcgagc tcggtacctt
     2761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     2821 gactggaagg gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat
     2881 aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc
     2941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     3121 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     3181 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     3241 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     3301 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     3361 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     3421 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     3481 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     3541 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     3601 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     3661 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3721 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3781 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3841 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3901 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4741 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5461 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5821 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5881 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5941 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6001 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6061 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6121 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6181 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6241 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     6301 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     6361 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     6421 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     6481 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     6541 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     6601 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     6661 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     6721 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     6781 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     6841 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
     6901 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
     6961 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
     7021 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
     7081 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
     7141 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
     7201 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
     7261 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
     7321 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     7381 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     7441 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     7501 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     7561 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     7621 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     7681 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     7741 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     7801 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     7861 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     7921 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     7981 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     8041 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8101 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     8161 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     8221 acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     8281 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
     8341 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     8401 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     8461 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     8521 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     8581 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     8641 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     8701 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     8761 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8821 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8881 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8941 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9001 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9061 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     9121 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     9181 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     9241 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9301 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9361 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     9421 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     9481 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     9541 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     9601 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9661 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     9721 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     9781 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     9841 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9901 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9961 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    10021 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    10081 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    10141 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    10201 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    10261 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    10321 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
    10381 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
    10441 aaacaaatta caaaaattca aaattttccg atcacgagac tagcctcgac aagcttgatc
    10501 gatggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
    10561 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
    10621 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
    10681 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtgtcg
    10741 tgacgcgcat ccaggcctaa gcttacgc
//
