LOCUS       sequence-73651-e       10738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10738)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 10738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305590110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-9- 9"; volume: "110"; issue: "28"; pages: "11284-9"
COMMENT     Sequence Label: pWPT-CCC/ACC-mEGFP-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..10738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             46..756
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(85..106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(346..365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     693..714
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(792..1366,1367..1369,1370..1378)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(959..976)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1186..1205
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1387..1396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1393..2103
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1538..1556)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1806..1825
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2116..2704
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2169..2189)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2587..2598)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2849..2882)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2902..3082
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3107..3125)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3108..3125)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3415..3434)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3648..3667)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3767..3789
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3827..3845)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3913..4017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4018..4086,4087..4878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4236..4255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5049..5637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5538..5557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5791..5808
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5878..5898)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5883..6212
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6063..6198
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7346..7411
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7541..7561
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7986..8120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8023..8042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8077..8096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8289..8922
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8969..9094
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9591..9824
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10009..10053
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10320..10437
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10493..10704
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tcgagtaact aactaaccca tgggttgatt accatgggcg aattcagcaa gggcgaggag
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
      661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      721 gccgggatca ctctcggcat ggacgagctg tacaagttcg aattcgcatg catctagggc
      781 ggccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
      841 taaggccggt gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat
      901 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
      961 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     1021 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     1081 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     1141 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     1201 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     1261 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     1321 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg
     1381 ggatccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     1441 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     1501 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     1561 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     1621 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     1681 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     1741 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1801 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1861 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1921 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1981 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     2041 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     2101 taagcggccg ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2161 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2221 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2281 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2341 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2401 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2461 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     2521 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2581 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2641 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2701 ccgcatcggg caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     2761 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     2821 gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt atacgaagtt
     2881 atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3061 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc
     3121 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     3181 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     3241 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     3301 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     3361 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     3421 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     3481 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     3541 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     3601 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     3661 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3721 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3781 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3841 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4021 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4081 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4141 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4201 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4261 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4321 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4381 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4441 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4501 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4561 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4621 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4681 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4741 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4801 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4861 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4921 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4981 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5041 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5101 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5161 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5221 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5281 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5341 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5401 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     5461 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5521 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5581 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5641 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5701 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5761 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     5821 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
     5881 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5941 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6001 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6061 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6121 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6181 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
     6241 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
     6301 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
     6361 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
     6421 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
     6481 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
     6541 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     6601 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     6661 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
     6721 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
     6781 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
     6841 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     6901 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     6961 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     7021 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
     7081 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     7141 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt
     7201 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag
     7261 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     7321 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     7381 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     7441 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     7501 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     7561 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     7621 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     7681 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     7741 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     7801 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     7861 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     7921 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     7981 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8041 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8101 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     8161 ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt
     8221 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     8281 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     8341 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     8401 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     8461 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     8521 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     8581 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     8641 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     8701 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     8761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8881 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     8941 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     9001 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     9061 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     9121 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     9181 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     9241 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     9301 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     9361 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     9421 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9481 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9541 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9601 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9661 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9721 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9781 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     9841 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     9901 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     9961 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    10021 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    10081 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    10141 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    10201 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    10261 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
    10321 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10381 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttccg
    10441 atcacgagac tagcctcgac aagcttgatc gatggctccg gtgcccgtca gtgggcagag
    10501 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
    10561 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
    10621 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
    10681 aacgggtttg ccgccagaac acaggtgtcg tgacgcgcat ccaggcctaa gcttacgc
//
