LOCUS       sequence-73655-e       10724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305590110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-9- 9"; volume: "110"; issue: "28"; pages: "11284-9"
COMMENT     Sequence Label: pWPT-/CGA-mEGFP-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..10724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             32..742
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(71..92)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(332..351)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     679..700
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(778..1352,1353..1355,1356..1364)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(945..962)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1172..1191
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1373..1382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1379..2089
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1524..1542)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1792..1811
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2102..2690
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2155..2175)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2573..2584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2835..2868)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2888..3068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3093..3111)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3094..3111)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3401..3420)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3634..3653)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3753..3775
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3813..3831)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3899..4003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4004..4072,4073..4864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4222..4241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5035..5623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5524..5543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5777..5794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5864..5884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5869..6198
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6049..6184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7332..7397
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7527..7547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7972..8106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8009..8028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8063..8082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8275..8908
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8955..9080
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9577..9810
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9995..10039
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10306..10423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10479..10690
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tcgagtaact aactaacgaa tgggcgaatt cagcaagggc gaggagctgt tcaccggggt
       61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      181 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      241 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      481 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
      661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      721 cggcatggac gagctgtaca agttcgaata agcatgcatc tagggcggcc aattccgccc
      781 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
      841 gtttgtctat atgtgatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
      901 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
      961 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     1021 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     1081 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     1141 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     1201 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     1261 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     1321 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccgggat ccgccaccat
     1381 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     1441 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1501 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1561 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1621 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1681 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1741 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1801 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1861 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1921 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1981 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     2041 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgccga
     2101 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2161 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2221 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2281 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2341 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2401 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2461 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     2521 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2581 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2641 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggcaat
     2701 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2761 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc
     2821 aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc
     2881 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3061 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     3121 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     3181 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     3241 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     3301 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     3361 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     3421 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     3481 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     3541 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     3601 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3661 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3721 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3781 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3841 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4021 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4081 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4141 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4201 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4261 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4321 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4381 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4441 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4501 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4561 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4621 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4681 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4741 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4801 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4861 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5161 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5401 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
     5461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5641 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5701 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5761 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5821 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     5881 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5941 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6001 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6061 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6121 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6181 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     6241 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     6301 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     6361 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     6421 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     6481 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
     6541 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     6601 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     6661 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
     6721 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     6781 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     6841 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     6901 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     6961 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     7021 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
     7081 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
     7141 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
     7201 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
     7261 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     7321 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     7381 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     7441 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
     7501 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     7561 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     7621 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     7681 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
     7741 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
     7801 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
     7861 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     7921 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     7981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8101 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     8161 attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt cctctgaatt
     8221 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     8281 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     8341 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     8401 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     8461 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     8521 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     8581 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     8641 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     8701 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8881 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8941 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     9001 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     9061 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     9121 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     9181 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     9241 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     9301 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     9361 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     9421 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     9481 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     9541 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     9601 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     9661 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     9721 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     9781 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9841 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9901 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9961 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    10021 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    10081 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    10141 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    10201 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    10261 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
    10321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    10381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttccgatca cgagactagc
    10441 ctcgacaagc ttgatcgatg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
    10501 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
    10561 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
    10621 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
    10681 cagaacacag gtgtcgtgac gcgcatccag gcctaagctt acgc
//
