LOCUS       sequence-73666-e        1826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression Vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Hopkins BD, Fine B, Steinbach N, Dendy M, Rapp Z, Shaw J, Pappas K, 
            Yu JS, Hodakoski C, Mense S, Klein J, Pegno S, Sulis ML, Goldstein 
            H, Amendolara B, Lei L, Maurer M, Bruce J, Canoll P, Hibshoosh H, 
            Parsons R
  TITLE     A secreted PTEN phosphatase that enters cells to alter signaling and
            survival.
  JOURNAL   Science. 2013 Jul 26;341(6144):399-402. doi: 
            10.1126/science.1234907. Epub 2013 Jun 6.
  PUBMED    23744781
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1234907"; journalName: "Science"; date: 
            "2013-07-26- 26"; volume: "341"; issue: "6144"; pages: "399-402"
COMMENT     Sequence Label: JpExpress404 PTEN-LONG
FEATURES             Location/Qualifiers
     source          1..1826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1749..1790
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1800..1817
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aggaggtaaa acatatgagc gagtccccag tcaccatcag cagagcaggc aacgcaggtg
       61 aactggttag cccgttgctg ctgccgccga cgcgccgtcg tcgtcgccgt cacattcagg
      121 gtcctggtcc ggtgctgaac ctgccgtctg ctgcagctgc tccgccggtt gctcgtgcgc
      181 cagaggcagc gggtggcggt tcccgttccg aggattatag ctcctcgccg catagcgcag
      241 cagcggcagc tcgtccgctg gcggcagagg aaaagcaggc tcagtcgctg caaccgagct
      301 ctagccgtcg ttctagccac tacccagcgg ccgttcagag ccaggcagcc gcagaacgtg
      361 gcgcgagcgc gacggccaaa agccgcgcaa ttagcattct gcaaaagaaa ccgcgtcacc
      421 aacaactgtt gccaagcttg agcagcttct ttttcagcca ccgtctgccg gatatgaccg
      481 cgattatcaa agagatcgtg tcccgtaaca agcgccgtta tcaagaagat ggctttgacc
      541 tggacctgac ctacatctac ccgaacatta tcgcgatggg ttttccagcc gagcgcctgg
      601 agggcgtcta ccgtaataac atcgatgatg tggttcgttt tctggacagc aaacataaga
      661 accattacaa gatctataat ctgtgcgccg agcgtcatta tgacaccgcg aaattcaatt
      721 gccgtgttgc gcagtacccg ttcgaggacc acaatccgcc gcaactggaa ctgattaaac
      781 cgttctgcga ggacctggat cagtggctgt ccgaggacga caaccacgtg gccgcgattc
      841 actgtaaggc cggcaaaggt cgtaccggcg tcatgatctg cgcgtacctg ctgcaccgcg
      901 gtaagtttct gaaggcgcaa gaagcgctgg acttttacgg cgaggtgcgt acccgcgaca
      961 aaaagggtgt caccatcccg agccaacgcc gctatgttta ctactacagc tatttgctga
     1021 aaaaccacct ggattatcgt cctgtcgccc tgttgtttca caagatgatg tttgaaacga
     1081 ttccgatgtt cagcggcggt acctgtaatc cgcagttcgt cgtttgtcag ctgaaggtga
     1141 agatctacag ctctaatagc ggtccgaccc gtcgcgaaga taagtttatg tatttcgaat
     1201 tcccgcagcc gctgccggtt tgtggtgaca tcaaggtgga gttcttccat aaacaaaaca
     1261 agatgttgaa gaaagataag atgttccatt tttgggtcaa cactttcttt attccgggtc
     1321 cggaggagac tagcgagaaa gttgagaacg gtagcctgtg cgatcaagaa atcgatagca
     1381 tttgcagcat cgaacgtgcg gataatgaca aagaatatct ggtcttgacc ctgacgaaaa
     1441 acgacctgga caaagcgaac aaagataaag cgaatcgcta tttcagcccg aatttcaaag
     1501 ttaagctgta ctttaccaaa actgtggagg aaccgagcaa tccggaggcg tctagctcta
     1561 ccagcgtgac cccggacgtc agcgacaatg agccggacca ctatcgttac agcgacacga
     1621 ccgacagcga tcctgaaaat gaaccgtttg acgaagatca acatacgcag attaccaagg
     1681 tgaaaggcaa cagcgcggat attcagcatt ctggtggccg tagcagcttg gaaggccctc
     1741 gcttcgaggg taagccgatt ccgaatccgc tgctgggtct ggatagcacg cgcacgggtc
     1801 accaccacca tcaccactaa ctcgag
//
