LOCUS       sequence-73676-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
  TITLE     RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
  PUBMED    23360965
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508."
COMMENT     Sequence Label: pCas9
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   389..424
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   455..490
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(491..622)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 gatgcgtccg gcgtagagga tccacaggac gggtgtggtc gccatgatcg cgtagtcgat
       61 agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc
      121 tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata
      181 gtgactggcg atgctgtcgg aatggacgat cacactactc ttcttttgcc tattataaca
      241 tgaaaacgct ttaacagaaa gaataggaag gtatccgact gctggtatta accctctttc
      301 tcaagttatc atcggcaatg ttgaatggag tccattcaaa acagcatagc tctaaaacat
      361 ggaactcaac aagtctcagt gtgctgaagt tttgggacca ttcaaaacag catagctcta
      421 aaacgagacc tttgagcttc cgagactggt ctcagttttg ggaccattca aaacagcata
      481 gctctaaaac ctcgtagact atttttgtct aaaaaatttc gtaatcgcac tatttgtctc
      541 agctagactt cagtcttgaa aagcccctgt attactgcat ttattaagag tattatacca
      601 tatttttagt tattaagaaa taatcttcat ctaaaatata cttcagtcac ctcctagctg
      661 actcaaatca atgcgtgttt cataaagacc agtgatggat tgatggataa gagtggcatc
      721 taaaacttct tttgtagacg tatatcgttt acgatc
//
