LOCUS       sequence-73677-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
  TITLE     RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
  PUBMED    23360965
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508."
COMMENT     Sequence Label: pCas9
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(85..90,91..107,108..113)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(431..509)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
       61 cgatataagt tgtaattctc atgtttgaca gcttatcatc gataagcttt aatgcggtag
      121 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct
      181 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt
      241 actgccgggc ctcttgcggg attacgaaat catcctgtgg agcttagtag gtttagcaag
      301 atggcagcgc ctaaatgtag aatgataaaa ggattaagag attaatttcc ctaaaaatga
      361 taaaacaagc gttttgaaag cgcttgtttt tttggtttgc agtcagagta gaatagaagt
      421 atcaaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt
      481 taacttgcta tgctgttttg aatggttcca acaagattat tttataactt ttataacaaa
      541 taatcaagga gaaattcaaa gaaatttatc agccataaaa caatacttaa tactatagaa
      601 tgataacaaa ataaactact ttttaaaaga attttgtgtt ataatctatt tattattaag
      661 tattgggtaa tattttttga agagatattt tgaaaaagaa aaattaaagc atattaaact
      721 aatttcggag gtcattaaaa ctattattga aatcatcaaa ctcattatgg atttaattta
      781 aactttttat tttaggaggc aaaaatggat aagaaatact caataggctt agatatcggc
      841 acaaatagcg t
//
