LOCUS       sequence-73678-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
  TITLE     RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
  PUBMED    23360965
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508."
COMMENT     Sequence Label: pCas9
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgttcagctt tcgttaaatt atcaaactta cgttgagtga ttaacttggc gtttagaagt
       61 tgtctccaat agtttttcat ctttttgact acttcttcac ttggaacgtt atccgattta
      121 ccacgatttt tatcagaacg cgttaagacc ttattgtcta ttgaatcgtc tttaaggaaa
      181 ctttgtggaa caatgtgatc gacatcataa tcacttaaac gattaatatc taattcttgg
      241 tccacataca tgtctcttcc attttggaga taatagagat agagcttttc attttgcaat
      301 tgagtatttt caacaggatg ctctttaaga atctgacttc ctaattcttt gataccttct
      361 tcgattcgtt tcatacgctc tcgcgaattt ttctggccct tttgagttgt ctgattttca
      421 cgtgccattt caataacgat attttctggc ttatgccgcc ccattacttt gaccaattca
      481 tcaacaactt ttacagtctg taaaatacct tttttaatag cagggctacc agctaaattt
      541 gcaatatgtt catgtaaact atcgccttgt ccagacactt gtgctttttg aatgtcttct
      601 ttaaatgtca aactatcatc atggatcagc tgcataaaat tgcgattggc aaaaccatct
      661 gatttcaaaa aatctaatat tgttttgcca gattgcttat ccctaatacc attaatcaat
      721 tttcgagaca aacgtcccca accagtataa cggcgacgtt taagctgttt catcacctta
      781 tcatcaaaga ggtgagcata tgttttaagt ctttcctcaa tcatctccct atcttca
//
