LOCUS       sequence-73687-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Flag tagged Eya2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  TITLE     Heide Ford plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 Flag-Eya2
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      144..153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             153..176
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc
      121 actagtccag tgtggtggaa ttcgccacca tggactacaa agacgatgac gacaaggtag
      181 aactagtgat ctcacccagc ctcactgtaa acagcgattg tctggataaa ctgaagttta
      241 accgtgctga cgctgctgtg tggactctga gtgacagaca aggcatcacc aaatcggccc
      301 ccctgagagt gtcccagctc ttctccagat cttgcccacg tgtcctcccc cgccagcctt
      361 ccacagccat ggcagcctac ggccagacgc agtacagtgc ggggatccag caggctaccc
      421 cctatacagc ttacccacct ccagcacaag cctatggaat cccttcctac agcatcaaga
      481 cagaagacag cttgaaccat tcccctggcc agagtggatt cctcagctat ggctccagct
      541 tcagcacctc acccactgga cagagcccat acacctacca gatgcacggc acaacagggt
      601 tctatcaagg aggaaatgga ctgggcaacg cagccggttt cgggagtgtg caccaggact
      661 atccttccta ccccggcttc ccccagagcc agtaccccca gtattacggc tcatcctaca
      721 accctcccta cgtcccggcc agcagcatct gcccttcgcc cctctccacg tccacctacg
      781 tcctccagga ggcat
//
