LOCUS       sequence-73689-e       10087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10087)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6-MBP TEV Ura S. cerevisiae express...
FEATURES             Location/Qualifiers
     source          1..10087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(704..722)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        790..894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(895..963,964..1755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1113..1132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1926..2514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2415..2434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2668..2685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2700..2840
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2766..2785)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2926..2948
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2942..3133)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        4551..5215
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    4767..4884
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5179..5202
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5249..5266
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5276..6376
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     6350..6373
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             6431..6451
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(7480..8283)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8284..8499)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      join(9091..10087,1..346)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(join(10054..10087,1..14))
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
       61 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
      121 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
      181 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
      241 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
      301 gattagtctc atccttcaat gctatcattt cctttgatat tggatcatat gcatagtacc
      361 gagaaactag tgcgaagtag tgatcaggta ttgctgttat ctgatgagta tacgttgtcc
      421 tggccacggc agaagcacgc ttatcgctcc aatttcccac aacattagtc aactccgtta
      481 ggcccttcat tgaaagaaat gaggtcatca aatgtcttcc aatgtgagat tttgggccat
      541 tttttatagc aaagattgaa taaggcgcat ttttcttcaa agctttattg tacgatctga
      601 ctaagttatc ttttaataat tggtattcct gtttattgct tgaagaattg ccggtcctat
      661 ttactcgttt taggactggt tcagaattct tgaagacgaa agggcctcgt gatacgccta
      721 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      781 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      841 ctcatgagac aataaccctg ataaatgctt caataataat gaaaaaggaa gagtatgagt
      901 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      961 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1021 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1081 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     1141 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1201 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1261 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1321 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1381 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     1441 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1501 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1561 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1621 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1681 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1741 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1801 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1861 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1921 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1981 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2041 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2101 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2161 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2221 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2281 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2581 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2641 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2701 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     2761 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     2821 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2881 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2941 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     3001 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     3061 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     3121 cctgtttggt cacttgatgc ctccgtgtaa gggggaattt ctgttcatgg gggtaatgat
     3181 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     3241 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     3301 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     3361 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     3421 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     3481 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     3541 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     3601 tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc
     3661 gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt
     3721 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
     3781 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
     3841 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg
     3901 acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc
     3961 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
     4021 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac
     4081 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg
     4141 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     4201 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     4261 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     4321 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     4381 ctcaagggca tcggtcgacc aattcgacag gttatcagca acaacacagt catatccatt
     4441 ctcaattagc tctaccacag tgtgtgaacc aatgtatcca gcaccacctg taaccaaaac
     4501 aattttagaa gtactttcac tttgtaactg agctgtcatt tatattgaat tttcaaaaat
     4561 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc
     4621 accatataca tatccatata catatccata tctaatctta cttatatgtt gtggaaatgt
     4681 aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct
     4741 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc
     4801 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga
     4861 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt
     4921 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat
     4981 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc
     5041 agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact gcataaccac
     5101 tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat gtaataaaag
     5161 tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa aaaaccccgg
     5221 atccgtaacc atgtcaactg gttcttctca ccatcaccat caccatggtt cttctatgaa
     5281 aatcgaagaa ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc
     5341 tgaagtcggt aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga
     5401 taaactggaa gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt
     5461 ctgggcacac gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc
     5521 ggacaaagcg ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg
     5581 caagctgatt gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct
     5641 gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc
     5701 gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat
     5761 tgctgctgac gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt
     5821 gggcgtggat aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa
     5881 caaacacatg aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga
     5941 aacagcgatg accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa
     6001 ttatggtgta acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt
     6061 gctgagcgca ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga
     6121 aaactatctg ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc
     6181 cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat
     6241 ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta
     6301 tgccgtgcgt actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct
     6361 gaaagacgcg cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct
     6421 cgggatcgag gaaaacctgt acttccaatc caatattgga agtggataac tctcgaggat
     6481 cgtaatgact agattatata atcagaaata gcctttggtg atcacttcca ttttagtcag
     6541 cataatagaa agtttaatta cgcgcttttt tacattccga caaagatctg cccgagaaaa
     6601 ttcttcgaga agacaacaac aacaaaaaaa ataaagcctg ggcgaaaaac atcgtgcgtt
     6661 tacataatat cttgaaaagc aaacagatat ataaaccgct attgaaatta cctttttaag
     6721 tattcgccta gaagccttgt ttcaagtagc atcaacaaca ggtgttaggt taaactgctt
     6781 tgactttttg ttctttaacg aatatcatta aaaaaaaagg acatctataa agaatgtctc
     6841 caaactcgtc gaaaactcga acggaccaga taagctccat gcctggtatt aatgaagcca
     6901 cgaaggtgga aagtaagaat gttgtgaagg atgctgttcc aataaaatct gaagttgaaa
     6961 ccaatggtac atcaatagta agagaaaaac aagatccaca ggacgggtgt ggtcgccatg
     7021 atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag
     7081 cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct
     7141 agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc
     7201 cggcagtacc ggcataacca agcctatgcc tacagccttc caggtgacgg tgccgagatt
     7261 gaccatgaac gccttgttag atttctacac cggtgcctga ctgcgttagc aatttaactg
     7321 tgataaacta ccgcattaaa gctttttctt tccaattttt tttttttcgt cattataaaa
     7381 atcattacga ccgagattcc cgggtaataa ctgatataat taaattgaag ctctaatttg
     7441 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     7501 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     7561 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     7621 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     7681 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     7741 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     7801 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     7861 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     7921 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     7981 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     8041 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     8101 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     8161 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     8221 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     8281 catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     8341 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     8401 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     8461 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa agcttatcga tgataagctg
     8521 tcaaacatga gaattccacg gactatagac tatactagta tactccgtct actgtacgat
     8581 acacttccgc tcaggtcctt gtcctttaac gaggccttac cactcttttg ttactctatt
     8641 gatccagctc agcaaaggca gtgtgatcta agattctatc ttcgcgatgt agtaaaacta
     8701 gctagaccga gaaagagact agaaatgcaa aaggcacttc tacaatggct gccatcatta
     8761 ttatccgatg tgacgctgca gcttctcaat gatattcgaa tacgctttga ggagatacag
     8821 cctaatatcc gacaaactgt tttacagatt tacgatcgta cttgttaccc atcattgaat
     8881 tttgaacatc cgaacctggg agttttccct gaaacagata gtatatttga acctgtataa
     8941 taatatatag tctagcgctt tacggaagac aatgtatgta tttcggttcc tggagaaact
     9001 attgcatcta ttgcataggt aatcttgcac gtcgcatccc cggttcattt tctgcgtttc
     9061 catcttgcac ttcaatagca tatctttgtt aacgaagcat ctgtgcttca ttttgtagaa
     9121 caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca
     9181 gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg
     9241 taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     9301 ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt
     9361 ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta
     9421 ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg
     9481 tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct
     9541 gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata
     9601 aaggcatccc cgattatatt ctataccgtc acaaagtatg cgcaatccac atacagaaag
     9661 tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc
     9721 tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga
     9781 atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg
     9841 tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg
     9901 gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta
     9961 ttcgcattat tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt
    10021 cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag
    10081 gaacttc
//
