LOCUS       sequence-73694-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of Eya2 and YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     Heide Ford plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MSCV-Eya2
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(141..143,144..146,147..659)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(186..207)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(447..466)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
       61 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
      121 atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      181 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      241 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      301 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc
      361 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gagggctacg
      421 tccaggagcg caccatctcc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      481 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      541 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      601 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      661 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      721 tgctgctgcc cgacaaccac tacctgagct accagagcgc cctgagcaaa gaccccaacg
      781 agaagcgcga tcacatggt
//
